Description
The RefSeq mRNAs gene track for the 27 Nov 2022 Harpia harpyja/GCF_026419915.1_bHarHar1_primary_haplotype
genome assembly displays translated blat alignments of vertebrate and
invertebrate mRNA in
GenBank.
Data Access
Download GCF_026419915.1_bHarHar1_primary_haplotype.xenoRefGene.gtf.gz GTF file.
Track statistics summary
Total genome size: 1,351,447,071
Gene count: 13,412
Bases in genes: 476,133,537
Percent genome coverage: % 35.231
Methods
The mRNAs were aligned against the Harpia harpyja/GCF_026419915.1_bHarHar1_primary_haplotype genome using
translated blat. When a single mRNA aligned in multiple places, the alignment
having the highest base identity was found. Only those alignments having a base
identity level within 1% of the best and at least 25% base identity with the
genomic sequence were kept.
Specifically, the translated blat command is:
blat -noHead -q=rnax -t=dnax -mask=lower target.fa query.fa target.query.psl
where target.fa is one of the chromosome sequence of the genome assembly,
and the query.fa is the mRNAs from RefSeq
The resulting PSL outputs are filtered:
pslCDnaFilter -minId=0.35 -minCover=0.25 -globalNearBest=0.0100 -minQSize=20
-ignoreIntrons -repsAsMatch -ignoreNs -bestOverlap
all.results.psl GCF_026419915.1_bHarHar1_primary_haplotype.xenoRefGene.psl
The filtered GCF_026419915.1_bHarHar1_primary_haplotype.xenoRefGene.psl is converted to
genePred data to display for this track.
Credits
The mRNA track was produced at UCSC from mRNA sequence data
submitted to the international public sequence databases by
scientists worldwide.
References
Benson DA, Cavanaugh M, Clark K, Karsch-Mizrachi I, Lipman DJ, Ostell J, Sayers EW.
GenBank.
Nucleic Acids Res. 2013 Jan;41(Database issue):D36-42.
PMID: 23193287; PMC: PMC3531190
Benson DA, Karsch-Mizrachi I, Lipman DJ, Ostell J, Wheeler DL.
GenBank: update.
Nucleic Acids Res. 2004 Jan 1;32(Database issue):D23-6.
PMID: 14681350; PMC: PMC308779
Kent WJ.
BLAT - the BLAST-like alignment tool.
Genome Res. 2002 Apr;12(4):656-64.
PMID: 11932250; PMC: PMC187518
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